Next-Generation Sequencing (NGS)
Over the recent years, the high-throughput next-generation sequencing (NGS) technology has risen as an indispensable instrument for the field of biomedicine, producing an unrivalled volume of sequence data.
For optimizing your NGS-driven research, we present unique solutions for NGS target enrichment, library preparation, and single-cell analysis. Each solution has been engineered to minimize bias and guarantee uniform coverage, allowing for more precise interpretation of biological samples.
Our streamlined 'easy to understand' protocols facilitate effective automation and seamless workflow integration, thereby accelerating data acquisition and analysis. This in turn enhances the utilization of the profound capabilities of next-generation sequencing technologies. Our ultimate objective is to support you in extracting maximum value from your research and achieving unparalleled outcomes.
In applying digital DNA sequencing (DNA-seq), the initial step for reliable outcomes centers on standardized sample coverage, combined with the capability to handle massive data quantities and scrutinize various internal discrepancies. Addressing these issues consistently demands substantial investment in sequencing capabilities and bioinformatics expertise. Notably, the data eminent from digital DNA sequencing execution may not sufficiently balance the additional expenses when juxtaposed with other tests, such as whole exome sequencing or targeted panel sequencing.
To approach this complexity, we've devised a specific DNA sequencing kit uniquely designed for targeted digital DNA sequencing. Assuring precise and dependable detection of low-frequency variants. In addition, our product line includes integrative multimodal resources for targeted DNA and RNA sequencing which promotes easier workflow management and assists in DNA variant enrichment and analysis, RNA fusions, and gene expression levels.
All our kits and solutions are subjected to strict validation and optimization processes to guarantee optimal variant detection while mitigating false positives. Our goal is to present you with a solution that is efficient, accurate, and cost-effective to meet the challenges in the field of bioinformatics.
You may explore our DNA sequencing products by accessing the links provided below.
RNA sequencing is a procedure based on second-generation sequencing technology that helps to quickly identify the type and amount of RNA in the genome. This includes a variety of RNA types, including messenger RNA and small RNA. this technology can examine different transcript forms of genes, gene fusions, post-transcriptional modifications, mutations or single nucleotide polymorphisms (SNPs), as well as variations in gene expression over time or differences in gene expression in different populations.
While lauding the transformative role that RNA sequencing technology has played in contributing to novel discoveries and insights, it is vital to remember that RNA molecules, specifically messenger RNA and ribosomal RNA, are highly vulnerable to their immediate environment and their handling techniques. These molecules can be affected by heat, ultraviolet radiation, and enzymatic contamination from ribonucleases. Consequently, their stability is an aspect that merits our utmost attention during the RNA sequencing process.
To aid in circumventing these hurdles, we offer a meticulously designed RNA sequencing kit. This toolkit is configured to maximize the safeguarding of your samples, thereby yielding more accurate findings. By utilizing state-of-the-art RNA sequencing technology, our kit can accurately identify and detect RNA molecules that are present in low quantities, critical for uncovering and decoding rare transcripts and genes that are lowly expressed.
Whole Genome/Transcriptome Amplification
Conducting concurrent whole-genome and whole-transcriptome amplifications serves to unify genomic and transcriptomic investigations, empowering investigators to concurrently survey DNA sequences encompassing the genome and RNA sequences constituting the transcriptome. Yet, acquiring an adequate quantity of specimens for scrutiny can pose difficulties. Compounding this, the amplification techniques implemented can incur errors, leading to biased samples and underrepresented genetic loci.
Fortunately, we provide bespoke whole genome and whole transcriptome amplification kits, designed to overcome these impediments. These kits leverage innovative amplification technologies that ensure exceptional uniformity in genome coverage and unparalleled sequence fidelity. Consequently, this limits the likelihood of false positives and missed detection events, enhancing the precision and dependability of analyses.
Our dedicated kits undergo stringent validation and optimization to curtail errors and biases, verifying accurate and consistent amplification outcomes. Moreover, they exhibit adaptability and can be modified to comply with a range of experimental prerequisites, contingent on varying scientific demands.
Metagenomic analysis refers to the genomic exploration of microbial ecologies within diverse specimen types including environmental, fecal, oral, and genitourinary samples. The goal of these studies is to elucidate the structure and functionality of these microbe communities across different specimens, and uncover their interspecies interactions. Crucial characteristics for these microbial investigations include high result yields and impartiality of results, which should remain consistent across all sample origins.
To meet these necessary conditions, we provide an array of tailored procedures designed for metagenomics applications. These programs are extraordinarily suitable for any metagenomics methodology, extending from 16S rRNA gene sequencing, to whole genome sequencing and whole transcriptome sequencing. We offer kits which cater to each stage of the procedure, including sample preparation, building of genetic libraries, sequencing reactions and computational data analysis.