20S Immunoproteasome (Human) (Untagged), ≥95% (SDS-PAGE)
Description
The 20S immunoproteasome is structurally similar to the constitutive 26S proteasome. The immunoproteasome 20S core contains 2 outer rings composed of α-subunits and 2 inner rings composed of 7-subunits, each ring containing 3 specific subunits responsible for the catalytic activity of the proteasome. In the immunoproteasome, these subunits (β1, β2, β5) are replaced by three inducible subunits: PSMB9, PSMB10 and PSMB8, (β1i, β2i, β5i). These stress-induced subunits allow the production of MHC-1-related peptides that are displayed as antigens on the cell surface. These displayed peptides can then be recognized by immune surveillance CD8 T cells. The 20S immunoproteasome is considered a powerful drug target in autoimmune diseases and cancer.
Basic Information
| Molecular Weight: | ~700kDa |
| Application: | Experiments involving the 20S immunoproteasome should be conducted using concentrations ranging from 2-5nM.The 20S immunoproteasome is typically found associated with regulatory complexes such as the 19S, PA28 α/β, or PA28γ. If the decision is made to exclude PA28 during the assay, the 20S immunoproteasome must be chemically activated by adding 0.035% SDS to the final assay buffers. |
| Concentration: | Lot dependent. |
| Crossreactivity: | Human |
| Formulation: | Liquid. In 50mM HEPES pH 7.5, 1mM TCEP, 100mM sodium chloride |
| Purity: | ≥95% (SDS-PAGE) |
| Sequence: | Human 20S Immunoproteasome. |
| Source/Host: | Human PBMCs |
Shipping and Handling
| Storage (Short Term): | -80°C |
| Storage (Long Term): | -80°C |
| Shipping: | Dry Ice |
| Handling Advice: | To prevent freeze/thaw cycles, it is recommended to prepare aliquots of the compound. |
| Stability: | When stored at a temperature of -80°C, it remains stable for at least 1 year. |
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