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5-Formylcytosines (5-fC) DNA Matched Antibody Pair
Description
5-Formylcytosine (5fC) is a modified pyrimidine nitrogen base derived from cytosine, and it is a recently discovered DNA modification. Similar to the generation of 5-hydroxymethylcytosine (5-hmC) by the action of the Tet family of cytosine oxygenase enzymes on 5-methylcytosine (5-mC), it has been observed that the continued activity of Tet proteins on hydroxymethylcytosine leads to the generation of 5-formylcytosine (5-fC) and 5-carboxylcytosine. This conversion process is believed to potentially represent a pathway for DNA demethylation.
Basic Information
| Synonyms: | 5-fC |
| Application: | Quantitative ELISA |
| Assay Type: | Sandwich |
| Crossreactivity: | All |
| Detection Type: | Colorimetric |
| Kit Contains: | 1) Capture Antibody: Anti-5-Formylcytosine (5-fC) Rabbit Monoclonal Antibody, Clone RM477, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins. 2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBSwith 0.09% sodium azide and 1% BSA |
| Quantity: | ELISA:Capture Antibody 50-200ng/wellDetection Antibody 0.1-0.5μg/mL |
Shipping and Handling
| Storage (Long Term): | -20°C |
| Shipping: | Blue Ice |
| Handling Advice: | To prevent freeze/thaw cycles, it is recommended to prepare aliquots of the compound. |
| Stability: | When stored at a temperature of -20°C, it remains stable for at least 1 year after receipt. |
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