5-Methylcytosine (5-Mc) DNA Matched Antibody Pair
Description
5-Methylcytosine is a methylated form of the DNA base cytosine that regulates gene transcription and is involved in epigenetics. It is one of the most well-known epigenetic modifications. This methylation usually occurs on the cytosines of vertebrate CpG dinucleotides. When cytosine is methylated, the DNA sequence remains unchanged, but the expression of the methylated genes changes. 5-Methylcytosine is a very important transcriptional repressor in the genome. When 5-methylcytosine is present in the promoter, it is associated with stable, long-term transcriptional silencing. This may occur by blocking positive transcription factors or by promoting the binding of negative transcription factors.
Basic Information
| Synonyms: | 5-mC |
| Application: | Quantitative ELISA |
| Assay Type: | Sandwich |
| Crossreactivity: | All |
| Detection Type: | Colorimetric |
| Kit Contains: | 1) Capture Antibody: Anti-5-Methylcytosine Rabbit Monoclonal Antibody, Clone RM231, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins. 2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide and 1% BSA. |
| Quantity: | ELISA:Capture Antibody 50 µg at 1.0mg/mLDetection Antibody 25 µg at 1.0mg/mL |
Shipping and Handling
| Storage (Long Term): | -20°C |
| Shipping: | Blue Ice |
| Handling Advice: | To prevent freeze/thaw cycles, it is recommended to prepare aliquots of the compound. |
| Stability: | When stored at a temperature of -20°C, it remains stable for at least 1 year after receipt. |
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