GeneRead DNAseq Gene Panel

The kit includes all the reagents necessary for targeted DNA sequencing except indexes. It also provides a custom panel designed for 96 samples.

Features

Pre-designed primers for gene design related to a disease or pathway are provided.
A simplistic protocol for various PCR instruments in the lab is available.
Amplification of essential exons is targeted. Analysis of sequence variation is available at no cost.
The product includes internal references for evaluating DNA quality and successful amplification before sequencing.

Product Details

The PROseq Targeted DNA Panels are designed as a comprehensive solution for digital DNA sequencing, which uses molecular barcodes. This unique methodology allows for the accurate detection of low-frequency variants, overcoming issues associated with PCR duplicates, false positives, and library bias.
Each DNA panel offered provides all essential components for building libraries from enriched genomic targets, with the convenience of a single box and compatibility across different NGS platforms. An innovative primer design is consumed based on a single primer extension strategy. In this way, each genomic target is enriched with a specific primer and a universal primer. This approach circumvents the limitation of traditional dual target-specific primer design, reducing the amount of primers needed. All necessary primers for a panel are combined into an individual primer pool, simplifying panel management and decreasing the number of pools needed for enrichment and library construction. The separate box of platform-specific indexes allows for multiplexing of up to 384 samples per sequencing run. The unique enzyme and buffer system in the PROseq targeted DNA panels provide optimal performance for high coverage of GC-rich genomic regions.
Panel customization is available to target gene exonic areas, hotspots or SNPs, as well as intronic and promoter regions.

Applications

The GeneRead DNAseq Gene Panels are ideal for enhancing genes linked to various biomedical fields, including cancer, genetic disorders, neurological disorders, cardiomyopathies, developmental disorders, metabolic disorders, and so on. These panels are utilized in identifying data about genetic alterations in the following NGS analyses.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.