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Human AADACL2 siRNA (RNAi)
Description
siRNA inhibits AADACL2 expression through RNA interference.
Product Data
| Component: | This product comes in either three 5 nmol vials (15 nmol) or two sets of three 5 nmol vials (30 nmol) of lyophilized siRNA oligo duplexes. Each vial contains slightly different sequences to completely knock out the gene. |
| Target: | AADACL2 |
| Species Reactivity: | Human |
| Tested Applications: | RNAi |
| Host: | Synthetic |
| Symbol: | AADACL2 |
| Form: | Lyophilized |
| Purity: | > 97% |
| Gene ID: | 344752 |
| UniProt ID: | Q6P093 |
| Specificity: | AADACL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligonucleotide duplex with target specificity that knocks down gene expression. |
| Application: | The duplexes can be individually transfected or combined to knock down the target gene, typically tested by qPCR or a western blot. |
| Shipping: | Shipped at 4 °C. |
| Storage: | Up to one year at -20 °C. |
Guidelines
|
1. Prior to resuspension, give the tube a quick spin in the centrifuge to make sure the freeze-dried siRNA settles at the bottom. 2. Stir the siRNA oligos into DEPC water until you reach the desired concentration. For instance, you can dissolve one vial of 5 nmol siRNA oligo in 250 µl of DEPC water to end up with a final concentration of 20 µM. 3. 48 to 72 hours before lysing the cells, transfect them with an amount of siRNA ranging from 10 nM to 100 nM. |
Quality Control
| The process of oligonucleotide synthesis is carefully tracked on a base by base basis using trityl analysis. This ensures the efficiency of the coupling process. After that, the oligonucleotide is purified by carrying out affinity-solid phase extraction. A mass spectrometer is used to further investigate the annealed RNA duplex, confirming the precise composition. Each batch is compared to the one before it using mass spectrometry to achieve the highest level of consistency between batches. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
