PROseq 16S/ITS Smart Control

The trial size kit comes with FastSelect Reagent, PROseq Beads, PROseq Bead Binding Buffer, Buffer FH and Nuclease Free Water.

Cat. No.	Product Name	Products Type	Tests	Datasheet	Price
NMTM-2312-HMS-1	PROseq 16S/ITS Smart Control		10	Datasheet	INQUIRY
NMTM-2312-HMS-2	PROseq 16S/ITS Region Panel		24/96	Datasheet	INQUIRY

Features

The removal of pan-bacterial 5S/16S/23S rRNA from fragmented or full-length RNA for metatranscriptomic studies.
A unique RNA removal method that doesn't rely on hybrid capture or enzymatic digestion.
For ribosomal RNA removal from human, mouse, and rat samples for host-microbe studies, combine with PROseq FastSelect -rRNA HMR.
Works with PROTHERAGEN, Illumina, NEB, and KAPA stranded RNA-seq library preparation kits.
In-silico design anticipates the blocking of over 95% of all 5S, 16S, and 23S rRNA database sequences.

Product Details

The PROseq FastSelect -5S/16S/23S Kits employs an original approach to eliminate the extremely abundant 5S/16S/23S rRNA from your RNA-seq library. This specific design caters to intricate community microbial samples drawn from soil, water, stool, and sludge that houses various and diverse bacterial populations. The coverage of rRNA given by this kit is drawn from SILVA 16S sequences (around 600,000 entries), SILVA 23S sequences (approximately 170,000 entries), and 5S rRNA database sequences (more than 7200 entries). In-silico modelling displays coverage exceeding 95% of all known 5S, 16S and 23S rRNA sequences.
Compatibility tests with PROTHERAGEN, Illumina and NEB RNA-seq library kits showed that PROseq FastSelect -5S/16S/23S works well with all leading RNA-seq library preparation methods. The easy-to-follow 14-minute protocol and bead cleanup can be smoothly integrated with all widespread RNA-seq library kits. It can be applied to either fragmented or full-length (non-fragmented) RNA.

Applications

PROseq FastSelect -5S/16S/23S provides quick and reliable removal of rRNA for bacterial isolates and complex bacterial communities.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.