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PROseq Low Input RNA Library Kit

The RNA-seq of RNA, which is isolated from the blood of human, mouse, rat, and other similar species, includes reagents for the preparation of RNA libraries for 24 samples. Additionally, it incorporates PROseq FastSelect -rRNA HMR and PROseq FastSelect -rRNA globin, which are used for the removal of cytoplasmic and mitochondrial ribosomal RNA as well as globin mRNA. PROseq Beads and PROseq Advanced Analysis are also included for the RNA-seq Analysis Portal.

Features

Initiate with 1-1000 ng of total RNA or enriched mRNA. Integrated PROseq FastSelect RNA removal reagent aids in eliminating ribosomal RNA or other not needed RNAs. Implementing sample barcoding after reverse transcription avoids any sample confusion. The package includes methods for library building for whole transcriptome or 3’ RNA-seq using total RNA or enriched mRNA. The package also provides access to online RNA-seq read mapping, differential gene expression, and pathway analysis for supported species via the RNA-seq Analysis Portal.

Product Details

PROseq FastSelect RNA Library Kits make use of a straightforward and quick procedure for holistic transcriptomics, commencing from 1 ng of total RNA. The FastSelect RNA removal reagents, incorporated into the uncomplicated workflow, is designed to eliminate ribosomal RNA and other undesired RNAs. This allows for the detection of gene expression and transcript from different model systems such as human, mouse, rat and related species, plants, yeast, zebrafish, flies, worms and bacteria. Additionally, researchers have the option to utilize mRNA enrichment kits (available separately) that enhance focus on the construction of RNA-seq library on protein coding mRNAs. By employing the PROseq UX Index IL UDI Index Kits, which are sold separately, it's possible to merge 768 separate samples into one single sequencing flow cell lane.

Applications

The PROseq FastSelect RNA Library Kits aid in the formation of stranded RNA-seq libraries, contributing to the identification of transcripts, discovery of fusion genes, and gene expression on a large scale. These kits can work with both high-quality and fragmented RNA, making it possible to construct RNA-seq libraries using tissues, exosomes, FFPE samples, and cell lines. Additionally, these kits also accept RNA from eukaryotic, prokaryotic and fungi samples.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.