PROseq miRNA Library Kit

Reagents for 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup, as well as quality control primers, are required for 12 sequencing prep reactions.

Features

The library construction time is shortened by the gel-free miRNA sequencing library prep.
The highest fidelity and most efficient data are achieved by eradicating adapter dimers and undesired RNA species.
The quantification of individual miRNA molecules is facilitated by integrated unique Molecular Indices (UMIs).
The package provides access to the RNA-seq Analysis Portal for samples of humans, mice, and rats.
It's also available with a staggering 768 unique dual indices specifically for high-throughput sequencing on Illumina NovaSeq instruments.

Product Details

Robust, miRNA-specific libraries can be achieved through optimized reaction chemistry, which also minimizes reaction biases and eliminates adapter dimers. Unique Molecular Indices (UMIs) can reduce PCR and sequencing bias by tagging each miRNA at an early stage. The GeneGlobe-integrated RNA-seq Analysis Portal simplifies miRNA-seq data analysis. This user-friendly, web-based data analysis tool is designed for biologists and is incorporated with PROseq Stranded RNA Library Kits.
768 unique dual indices now allow for high-throughput sequencing on Illumina NovaSeq instruments.
As an essential point, we strongly advise comparing data only with RNA-seq libraries utilizing the same type of indices (single or unique dual indices). This guarantees experimental consistency. Comparison of samples and data should be done within the same indexing type - single-end indexed libraries with single-end indices, and unique dual indices with unique dual indices.

Applications

The PROseq miRNA Library Kit functions with a multitude of biofluids including serum, plasma, CSF, and urine, as well as cells and both fresh/frozen and FFPE tissues that contain total and miRNA. Utilize this kit for profiling the upcoming circulating miRNA biomarker, exploring hidden miRNA signatures in FFPE tissues, and fully identifying a new species. The kit was designed specifically to boost productivity from biofluids, such as serum. The PROseq miRNA Library Kit's functionality is illustrated well in the figure "Detection of miRNA", showcasing a strong ability to detect miRNA in serum samples. The kit proved superior in mapping miRNAs, even under the limitation of samples (as shown in "Read distribution in serum samples"). This kit enables you to examine the variances in expressions of any known or new miRNAs from any total RNA sample from any species.
The PROseq miRNA operates as a powerful tool, augmenting discovery and expression from big-scale projects with hundreds of samples to the smaller, target-oriented pilot. With PROseq miRNA, experience an unparalleled Sample to Insight solution for different expression analysis and the unveiling of new miRNAs with the application of next-generation sequencing.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.