PROseq Single Cell DNA Library Kits UDI

This is for 24 reactions and includes buffers and reagents necessary for cell lysis, whole genome amplification, and library preparation, such as DNA fragmentation, end-repair, and adapter ligation. It also comprises PROseq Beads and a plate with 24 UDI barcoded adapters compatible with Illumina instruments.

Features

It's possible to obtain whole genome libraries originating from single cells in less than four hours.
You can examine variations in the sequence and the quantity of genetic copies anywhere across the genome.
These processes provide NGS libraries free from PCR and augmented gDNA which can be used in subsequent testing.
The REPLI-g DNA Polymerase MDA enhances the preciseness of the amplification and it lowers the level of false-positives.
This methodology includes a unique dual index system to reduce sequenced artifacts due to "index hopping" on Illumina NGS machines.

Product Details

The PROseq Single Cell DNA Library Kit UDI is a comprehensive solution equipped for whole genome sequencing from either single cells or limited quantities of genomic DNA. It comes complete with all necessary reagents for cell lysis, whole genome amplification through REPLI-g DNA polymerase, enzymatic DNA splitting and PCR-free NGS library creation compatible with Illumina NovaSeq and other NGS instruments. It offers broad genome coverage and exceptional sequence accuracy, reducing false positives and dropouts. It's well-suited to analyze aneuploidy, copy number and sequence variations in diverse cells from scarce samples due to its PCR-free approach. This kit includes PROseq Unique Dual-Index Y-Adapters and PROseq Beads for reaction cleanup and accommodates all necessary reagents for DNA library assembly. The capacity of the kit allows it to handle up to 384 samples in a single flow-cell lane, which optimizes sample throughput and reduces sequencing costs.
This kit also comes with instructions for unbiased whole genome amplification and for target enrichment of small genomes, such as viral DNA or mitochondrial genomes.

Applications

Analysis of small DNA genomes focused on specific targets Examining the heterogeneity of genomes between cells Detection of genetic mutations
Analysis related to variations in copy number
Evaluation of aneuploidy

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.