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PROseq Targeted DNA IO Panel

A set comprising of all reagents (excluding indexes) for targeted DNA sequencing and a pre-determined panel for 12 samples. It involves less than 100 genes.

Features

Digital sequencing is enabled by molecular barcodes which removes PCR duplicates. The complete sample to insight solution simplifies the workflow. The compatibility with low-quality DNA makes the efficient sequencing of FFPE and cfDNA samples possible. Precious samples are preserved through minimal DNA input. High coverage of GC-rich regions is achieved by optimized buffers and conditions.

Product Details

Each panel functions as a platform-independent, single-use NGS solution, containing all the necessary elements for building libraries from enriched genomic targets. The primer design is based on single primer extension, which involves the enrichment of each genomic target by one target-specific primer and one universal primer. This approach eliminates the conventional design restriction of two target-specific primers and reduces the primer quantity needed. All the primers necessary for a panel are pooled into a single primer pool to simplify the handling of the panel and lessen the number of required pools for enrichment and library construction. Besides, platform-specific indexes, stored in a separate box, enable the multiplexing of up to 384 samples in each sequencing run.

Applications

The PROseq targeted DNA panels can be utilized for identifying a variety of DNA variants from numerous types of samples, with wide applications. The DNA variants, include SNVs, small indels, and CNVs, while the range of samples feature FFPE, plasma/serum, fresh or frozen tissue, and cell lines. Its numerous applications include DNA variant profiling in solid and hematologic malignancies, hotspot detection in solid tumors, mitochondrial DNA variant examination, pain and ADME pharmacogenomics, human identity and paternity analysis, inherited diseases germline mutation assessment, and BRCA 1 and BRCA2 all exonic base profiling.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.