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Recombinant Human Insulin Protein
Description
After the signal peptide is removed, proinsulin undergoes post-translational cleavage to produce three peptides: the B chain and A chain peptides. These two peptides are then connected through two disulfide bonds to form insulin, along with the production of C-peptide. The interaction between insulin and the insulin receptor (INSR) triggers glucose uptake.
Basic Information
| Synonyms: | IDDM, IDDM1, IDDM2, ILPR, INS, IRDN, MODY10 |
| Application: | Cell Culture |
| Source/Host: | Yeast |
| Tag: | None |
| Mol_Mass: | 6 kDa |
| AP_Mol_Mass: | 6 kDa |
| Uniprot: | P01308 |
| Accession: | P01308 |
| Bio_Activity: | Measure the cell proliferation of MCF-7 human breast cancer cells using a serum-free assay. The effective dose (ED50) for this effect is typically 0.2-1 µg/mL. |
| Sequence: | Phe1-Lys29; Gly1-Asn21 |
| Species: | Human |
Product Properties
| Formulation: | Lyophilized from sterile PBS, pH 7.4. Prior to lyophilization, add protectants including 5% - 8% trehalose, mannitol, and 0.01% Tween80. |
| Purity: | > 95 % (by reducing SDS-PAGE). |
Shipping and Handling
| Shipping: | Shipped with ice packs (the product is lyophilized powder). |
| Stability & Storage: | Please store lyophilized proteins at temperatures between -20 to -80°C for a maximum of 12 months. Once reconstituted, the protein solution can be stored at temperatures ranging from 4-8°C for a period of 2-7 days. If you need to store aliquots of reconstituted samples, please ensure they are kept below -20°C and they will remain stable for up to 3 months. |
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
