REPLI-g Advanced DNA Single Cell Kit

Buffers, enzymes, and storage solutions for single cells are provided for 24 Whole Genome Amplification (WGA) reactions.

Cat. No.	Product Name	Products Type	Tests	Datasheet	Price
NWSCLI-2312-HMS-1	REPLI-g Advanced DNA Single Cell Kit		24/96	Datasheet	INQUIRY
NWSCLI-2312-HMS-2	REPLI-g Single Cell Cryo-Protect Reagent		15 mL	Datasheet	INQUIRY

Features

The WGA can successfully cover the complete genome from single eukaryotic cells or 1 ng genomic DNA.
The uncomplicated protocol, consisting of 3 steps, only needs 2 hours and 20 minutes to complete.
The procedure can consistently yield between 25-35 µg DNA from one eukaryotic cell.
The package includes a cryo-protect reagent that can diminish DNA damage during single cell capture, storage or shipping.

Product Details

The analysis of DNA sequences and genotyping of biological samples through next-generation sequencing (NGS) platforms often faces limitations due to the scarcity of the sample. The REPLI-g Advanced DNA Single Cell Kit, which is highly optimized for the sensitive applications of microarray and NGS, offers excellent uniformity and minimal allelic dropout when initiated with single cells or purified genomic DNA. A unique and controlled procedure is used to decontaminate buffers and reagents to decrease DNA contamination and ensure highly trustworthy results. The innovative Multiple Displacement Amplification (MDA) technology allows the precise amplification of genomes with negligible sequence bias and minimal genomic dropouts.

Applications

Sequencing of the entire genome
Sequencing of the exome
Genotyping arrays for single nucleotide polymorphisms (SNP) Array comparative genomic hybridization (CGH) Technologies involving quantitative PCR/PCR
Sanger sequencing
Pyrosequencing

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.