REPLI-g FFPE Kit

Buffers, Reagents and DNA Polymerase for whole genome amplification reactions of 25 x 50 μl.

Features

The entire genome can be amplified directly from paraffin sections.
DNA can be amplified across the entire genome from formalin-fixed paraffin-embedded (FFPE) tissues.
The DNA yield is scalable and standardized, producing up to 40 µg per tissue section.
The process is simple and rapid, providing quick results.

Product Details

Insufficient genomic DNA for genomic analysis can be addressed by amplifying the entire genomic DNA present in the sample. The REPLI-g FFPE Kit, equipped with DNA polymerase, innovative buffers, and reagents, can effectively amplify the entire genome from formalin-fixed, paraffin-embedded (FFPE) tissues without having to pre-purify the DNA. The process involves lysing the tissue sections, processing the DNA, ligation of DNA fragments and the subsequent amplification of the long strand DNA using REPLI-g technology.

Applications

The following applications are suitable for REPLI-g FFPE amplified DNA:
Real-time PCR, Endpoint PCR, SNP genotyping, Microsatellite analysis.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.