REPLI-g Single Cell Kit

The REPLI-g sc Polymerase, Buffers, and Reagents are designed for 24 whole genome amplification reactions, yielding up to 40 µg per reaction.

Features

Amplification of genomic loci can be carried out impartially using multiple substitution amplification technology.
This process is compatible with advanced technologies like second-generation sequencing.
It delivers stable yields as high as 40 µg, with an average product length greater than 10 kb.
It has applications in various fields of research such as cancer, stem cell research or metagenomics.

Product Details

Many researchers employ second-generation sequencing instruments for the analysis of DNA sequences and genotyping in biological samples, though the available sample size often limits them. The REPLI-g Single Cell Kit is utilized for the uniform amplification of single cells (ranging from 1 to 1000 such as bacterial or tumor cells) or purified genomic DNA, covering all genome loci. For dried blood, fresh or frozen tissue samples, supplementary protocols are provided. To ensure the validity of results for every experiment, all reagents and buffers are produced through a meticulously monitored process to avoid DNA contamination. The products use Multiple Displacement Amplification (MDA) technology to amplify all genomic loci without biases. This technology, compared to the PCR-based whole genome amplification, provides superior amplification while maintaining high fidelity and preventing incorrect positive or negative signals.

Applications

The DNA extracted through amplification with the REPLI-g Single Cell Kit can be utilized for numerous downstream applications. These include second-generation sequencing, SNP genotyping using TaqMan primer/probe pairs, mutation detection based on qPCR and PCR, STR/microsatellite analysis, Sanger sequencing, pyrophosphate sequencing, and aCGH microarray technologies.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.